Titration of anti TGFB1 antibody
Without stimulation
Titration plots for increasing doses of anti-TGFB1. Note the vertical line drawn at unstained (UU) and successive population shifts (non specific binding?). The ideal dose in this case must be close to 0.625 microL.
 |
| Doses ranging from 0.625 to 10 microL; note that entire cell population is moving |
Plotting median FITC versus dose shows a continuously increasing trend. so no help from there!
With antigenic (MTb) stimulation
Does not make much of a difference
(Antibody clone TW4-9E7 bound to Alexa fluor 488, detected on FITC channel; cells - healthy peripheral blood mononuclear cells).
No comments:
Post a Comment