A jar of snacks (namkeens) demonstrate the principles of column chromatography to a fairly accurate extent.
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| In a column consisting of differently sized particles, gravity arranges them over time to a particular configuration |
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| The smallest fragments gravitate to the bottom, followed by medium ones, until a gradient is formed from largest to smallest particles, to to bottom of the jar |
Imagine solving a puzzle with 100 pieces, each piece a centimeter in size, something like this:
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| The genome is considerably larger than this puzzle and has much more than 100 pieces to assemble, in a typical NGS run |
1. The size of each piece (a 'read' in NGS terminology) matters; larger pieces make solving the puzzle exponentially easier
2. A few pieces seem to fit in multiple places of the puzzle; they are similar to a great degree, (like the pieces containing black hair in this puzzle), however, differ ever so slightly (maybe in only a few pixels, or in NGS terms, nucleotides)
3. Some pieces are repetetive (like the 'blue sky' in this puzzle), which add to the difficulty of assembling the puzzle; they are equivalent to the nucleotide repeat sequences encountered in reads of NGS
4. In this puzzle, no two pieces overlap. However, NGS reads will often overlap with each other.
Together, these are the core issues in the genome assembly problem, i.e. reconstructing a genome from its fragments when you have no 'reference genome' to look up to.
Bankim Bhavan, Naihati
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| Aquarium at Science City Ahmedabad |
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| Some serious maintenance is due anyway |
Mumbai airport at night
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| The vietnamese paper that morning |
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| Lounge |
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| Shakerato at Hanoi |
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| A glimpse of Hanoi |
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| Five long hours at Hanoi |
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| Metro ticket from Incheon to Seoul |
Hotel Riviera, Cheongdam, Gangnam District, Seoul
Korean dinner at Todamgol
Insadong market, Seoul; kind of New Market in my city
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| These little 'music boxes' are spring operated; each plays one particular song, which can be 'programmed' by turning the spring in just the right way |
Gyeongbokgung Palace, one of the major regal palaces in Seoul
Myeongdong market Seoul; lots of facial masks, anti-ageing serum and glow-yourself-up creams. Still looking for the youth fountain
Ethics of AI in pathology symposium
Korean lunch with Quail's eggs, fish and invertebrates
Sejong university convention center; the center hosted the first congress of Asian Society of Digital Pathology
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The Chapel inside Sejong university
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| Statue for a song |
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| Next time you say you're buried under studies |
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| Yeah, well.. |
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| Bibimbap |
War memorial
National museum of Korea
Namsan trail
Bongeunsa temple; a monastery whack in the middle of Gangnam metropolis
A jar of snacks (namkeens) demonstrate the principles of column chromatography to a fairly accurate extent. In a column consisting of diffe...
