Titration of FoxP3 (tagged with PE). Note the gradual and slow population shift over increasing doses of antibody. (UU - unstimulated unstained, US - unstimulated stained, SS - stimulated & stained)
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| As the dose of antibody is inreased, the entire helper T cell population is taking up the stain (which is unwanted behavior) |
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| There is no drop in median PE expression with doses upto 10 microL. This means there might be yet more antibody targets left to bind |
In this case, 2.5 miroL dose which gives good positive population (2.86%) without staining the negative population. So we select this dose.
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